other:Article Title: Climate and seed mass drive intraspecific variation in seed longevity in storage
Article Snippet: We watered the seeds with a 0.025% w/v gibberellic acid solution prepared in distilled water (Fisher Chemical, Vienna, Austria) to break dormancy, sealed the plates with transparent Parafilm, and placed them in a climate‐controlled Rumed cabinet with a 14 h light at 20°C/10 h dark at 10°C.
Incubation:
Recombinant:Article Title: Ketogenic diet as a metabolic vehicle enhancing the therapeutic efficacy of mebendazole and devimistat in juvenile syngeneic high-grade glioma.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Caspase − 3 Cell Signaling 9662; AB_331439 OGDH Abcam Ab137773; AB_2924873 Glutaminase C Abcam Ab262716 Ki67 Cell Signaling 12202; AB_2620142 Actin Cell Signaling 5125; AB_1903890 Chemicals, peptides, and recombinant proteins DMEM without sodium pyruvate Corning 10017CV DMEM (basal media) Sigma D-5030 FBS Atlas Biologicals F0500 Glutamine Sigma D-5796 Glucose Sigma G7021 MBZ Sigma M2523 DON Sigma D-2141 CPI613 AdooQ Biosciences A11786 DMSO Fisher Scientific BP231 D-luciferin Syd Lab MB102 Methanol Sigma 34860 2,5-dihydroxybenzoic acid matrix Sigma 85707 Acetonitrile Sigma 1000291000 Cell lysis buffer Cell Signaling Technology 9803 Falcon 96 well plate Corning 353072 24 well plates Thermo Scientific 130186 Bis-Tris gel (4–12%) Invitrogen NP0336 PVDF Biorad 1704156 TBST Thermo Scientific J77500-K2 Critical commercial assays Alanine transaminase assay Cayman Chemicals 700260 Deposited data Metabolomics data DOI for this project PR003000: https://doi.org/10.21228/ M8WK2K – Metabolomics Workbench: PR003000 Experimental models: Cell lines SF188 - human Sigma SCC282; CVCL_6948 VM-M3/Luc cell line – mouse Laboratory of Thomas Seyfried – CT-2A/Luc cell line – mouse Laboratory of Thomas Seyfried – Experimental models: Organisms/strains VM/DK Boston College ACF MGI:2162556 C57BL6/J Boston College ACF IMSR_JAX:000664 Software and algorithms SciLS lab software Bruker Daltonics Version 2025b Aura Software for in vivo bioluminescence acquisition and analysis Spectral Instraments Imaging (a Bruker company) Version 2.3.1 Other KetoGEN Medica Nutrition – Cell Reports Medicine 7, 102845, June 16, 2026 e1 ..
Lysis:Article Title: Ketogenic diet as a metabolic vehicle enhancing the therapeutic efficacy of mebendazole and devimistat in juvenile syngeneic high-grade glioma.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Caspase − 3 Cell Signaling 9662; AB_331439 OGDH Abcam Ab137773; AB_2924873 Glutaminase C Abcam Ab262716 Ki67 Cell Signaling 12202; AB_2620142 Actin Cell Signaling 5125; AB_1903890 Chemicals, peptides, and recombinant proteins DMEM without sodium pyruvate Corning 10017CV DMEM (basal media) Sigma D-5030 FBS Atlas Biologicals F0500 Glutamine Sigma D-5796 Glucose Sigma G7021 MBZ Sigma M2523 DON Sigma D-2141 CPI613 AdooQ Biosciences A11786 DMSO Fisher Scientific BP231 D-luciferin Syd Lab MB102 Methanol Sigma 34860 2,5-dihydroxybenzoic acid matrix Sigma 85707 Acetonitrile Sigma 1000291000 Cell lysis buffer Cell Signaling Technology 9803 Falcon 96 well plate Corning 353072 24 well plates Thermo Scientific 130186 Bis-Tris gel (4–12%) Invitrogen NP0336 PVDF Biorad 1704156 TBST Thermo Scientific J77500-K2 Critical commercial assays Alanine transaminase assay Cayman Chemicals 700260 Deposited data Metabolomics data DOI for this project PR003000: https://doi.org/10.21228/ M8WK2K – Metabolomics Workbench: PR003000 Experimental models: Cell lines SF188 - human Sigma SCC282; CVCL_6948 VM-M3/Luc cell line – mouse Laboratory of Thomas Seyfried – CT-2A/Luc cell line – mouse Laboratory of Thomas Seyfried – Experimental models: Organisms/strains VM/DK Boston College ACF MGI:2162556 C57BL6/J Boston College ACF IMSR_JAX:000664 Software and algorithms SciLS lab software Bruker Daltonics Version 2025b Aura Software for in vivo bioluminescence acquisition and analysis Spectral Instraments Imaging (a Bruker company) Version 2.3.1 Other KetoGEN Medica Nutrition – Cell Reports Medicine 7, 102845, June 16, 2026 e1 ..
Software:Article Title: Ketogenic diet as a metabolic vehicle enhancing the therapeutic efficacy of mebendazole and devimistat in juvenile syngeneic high-grade glioma.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Caspase − 3 Cell Signaling 9662; AB_331439 OGDH Abcam Ab137773; AB_2924873 Glutaminase C Abcam Ab262716 Ki67 Cell Signaling 12202; AB_2620142 Actin Cell Signaling 5125; AB_1903890 Chemicals, peptides, and recombinant proteins DMEM without sodium pyruvate Corning 10017CV DMEM (basal media) Sigma D-5030 FBS Atlas Biologicals F0500 Glutamine Sigma D-5796 Glucose Sigma G7021 MBZ Sigma M2523 DON Sigma D-2141 CPI613 AdooQ Biosciences A11786 DMSO Fisher Scientific BP231 D-luciferin Syd Lab MB102 Methanol Sigma 34860 2,5-dihydroxybenzoic acid matrix Sigma 85707 Acetonitrile Sigma 1000291000 Cell lysis buffer Cell Signaling Technology 9803 Falcon 96 well plate Corning 353072 24 well plates Thermo Scientific 130186 Bis-Tris gel (4–12%) Invitrogen NP0336 PVDF Biorad 1704156 TBST Thermo Scientific J77500-K2 Critical commercial assays Alanine transaminase assay Cayman Chemicals 700260 Deposited data Metabolomics data DOI for this project PR003000: https://doi.org/10.21228/ M8WK2K – Metabolomics Workbench: PR003000 Experimental models: Cell lines SF188 - human Sigma SCC282; CVCL_6948 VM-M3/Luc cell line – mouse Laboratory of Thomas Seyfried – CT-2A/Luc cell line – mouse Laboratory of Thomas Seyfried – Experimental models: Organisms/strains VM/DK Boston College ACF MGI:2162556 C57BL6/J Boston College ACF IMSR_JAX:000664 Software and algorithms SciLS lab software Bruker Daltonics Version 2025b Aura Software for in vivo bioluminescence acquisition and analysis Spectral Instraments Imaging (a Bruker company) Version 2.3.1 Other KetoGEN Medica Nutrition – Cell Reports Medicine 7, 102845, June 16, 2026 e1 ..
In Vivo:Article Title: Ketogenic diet as a metabolic vehicle enhancing the therapeutic efficacy of mebendazole and devimistat in juvenile syngeneic high-grade glioma.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Caspase − 3 Cell Signaling 9662; AB_331439 OGDH Abcam Ab137773; AB_2924873 Glutaminase C Abcam Ab262716 Ki67 Cell Signaling 12202; AB_2620142 Actin Cell Signaling 5125; AB_1903890 Chemicals, peptides, and recombinant proteins DMEM without sodium pyruvate Corning 10017CV DMEM (basal media) Sigma D-5030 FBS Atlas Biologicals F0500 Glutamine Sigma D-5796 Glucose Sigma G7021 MBZ Sigma M2523 DON Sigma D-2141 CPI613 AdooQ Biosciences A11786 DMSO Fisher Scientific BP231 D-luciferin Syd Lab MB102 Methanol Sigma 34860 2,5-dihydroxybenzoic acid matrix Sigma 85707 Acetonitrile Sigma 1000291000 Cell lysis buffer Cell Signaling Technology 9803 Falcon 96 well plate Corning 353072 24 well plates Thermo Scientific 130186 Bis-Tris gel (4–12%) Invitrogen NP0336 PVDF Biorad 1704156 TBST Thermo Scientific J77500-K2 Critical commercial assays Alanine transaminase assay Cayman Chemicals 700260 Deposited data Metabolomics data DOI for this project PR003000: https://doi.org/10.21228/ M8WK2K – Metabolomics Workbench: PR003000 Experimental models: Cell lines SF188 - human Sigma SCC282; CVCL_6948 VM-M3/Luc cell line – mouse Laboratory of Thomas Seyfried – CT-2A/Luc cell line – mouse Laboratory of Thomas Seyfried – Experimental models: Organisms/strains VM/DK Boston College ACF MGI:2162556 C57BL6/J Boston College ACF IMSR_JAX:000664 Software and algorithms SciLS lab software Bruker Daltonics Version 2025b Aura Software for in vivo bioluminescence acquisition and analysis Spectral Instraments Imaging (a Bruker company) Version 2.3.1 Other KetoGEN Medica Nutrition – Cell Reports Medicine 7, 102845, June 16, 2026 e1 ..
Imaging:Article Title: Ketogenic diet as a metabolic vehicle enhancing the therapeutic efficacy of mebendazole and devimistat in juvenile syngeneic high-grade glioma.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Caspase − 3 Cell Signaling 9662; AB_331439 OGDH Abcam Ab137773; AB_2924873 Glutaminase C Abcam Ab262716 Ki67 Cell Signaling 12202; AB_2620142 Actin Cell Signaling 5125; AB_1903890 Chemicals, peptides, and recombinant proteins DMEM without sodium pyruvate Corning 10017CV DMEM (basal media) Sigma D-5030 FBS Atlas Biologicals F0500 Glutamine Sigma D-5796 Glucose Sigma G7021 MBZ Sigma M2523 DON Sigma D-2141 CPI613 AdooQ Biosciences A11786 DMSO Fisher Scientific BP231 D-luciferin Syd Lab MB102 Methanol Sigma 34860 2,5-dihydroxybenzoic acid matrix Sigma 85707 Acetonitrile Sigma 1000291000 Cell lysis buffer Cell Signaling Technology 9803 Falcon 96 well plate Corning 353072 24 well plates Thermo Scientific 130186 Bis-Tris gel (4–12%) Invitrogen NP0336 PVDF Biorad 1704156 TBST Thermo Scientific J77500-K2 Critical commercial assays Alanine transaminase assay Cayman Chemicals 700260 Deposited data Metabolomics data DOI for this project PR003000: https://doi.org/10.21228/ M8WK2K – Metabolomics Workbench: PR003000 Experimental models: Cell lines SF188 - human Sigma SCC282; CVCL_6948 VM-M3/Luc cell line – mouse Laboratory of Thomas Seyfried – CT-2A/Luc cell line – mouse Laboratory of Thomas Seyfried – Experimental models: Organisms/strains VM/DK Boston College ACF MGI:2162556 C57BL6/J Boston College ACF IMSR_JAX:000664 Software and algorithms SciLS lab software Bruker Daltonics Version 2025b Aura Software for in vivo bioluminescence acquisition and analysis Spectral Instraments Imaging (a Bruker company) Version 2.3.1 Other KetoGEN Medica Nutrition – Cell Reports Medicine 7, 102845, June 16, 2026 e1 ..
Extraction:
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